Method did not work!!!
Eugh, went to bed late and then woke up 5 minutes late, therefore i was 5 min late to uni. Sigh! It was sunny as well, but then i was so tired i didn't notice.
Got to uni and the first thing i knew was that i had to move upstairs to do my experiments but wingkit got to stay cos he got there before me! GRRRRR!!!! poo u wingkit. So, after wasting 30 min getting my stuff upstairs and to get used to the surroundings i found that it was not so bad after all. I get to have my own spectophotometer, there were three centrifuge machines, lots of pipettes and lots of SPACES!!!! hehe, the only thing it lacked was the company. Ah well, to make it up, i went downstairs to get some more stuff but also to make Wingkit really guilty. Hehe, and i succeeded, mwuhahahaha!!!
Awww, but then i felt bad so i stopped and went back upstairs again to face the stupid method. I did the assay i did yesterday and there was no reaction at all!!! It was 12 then and was ordered by Derek to go have lunch and do another method in the afternoon. So i trouped off with Wingkit to have lunch outside. Wow, it was really warm. I went to have ice-cream as well. Tried to coax Wingkit to have some too, cos i was feeling like a pig, but that did not work. I also noticed how thin he actually is when he keeps complaining how FAT he is. Ok, if he is "fat", then i must be OBESE!!!!
After chatting to Chin and Basma for a few minutes, we went back upstairs to face our project again. groan*
This time i did the GST binding buffer assay. Purifying the protein from the bacteria and then react it with the substrate (dichrlor stuff) and glutathione. I did the reaction and then found out that it did not work either. I also managed to bugger up the concentration/volume/measurement/counting/timing of the stupid method. Sigh, dunno if it really mess my results that bad or not. Then after Derek came up to check up on me, i realised something....i had forgotten to put the glutathione into the Reconstitution buffer!!!! CRAP CRAP CRAP CRAP CRAP CRAP CRAP*
I spent the last hour redoing most of the method which includes long minutes of contrifuging and shaking the mixture for ages. I got so sleepy that i put my head on the table and shook the tube in one hand. Just at that point i heard the door open and Dr Archer came through the door. I bolted upright and gave a sheepish smile. He laughed at me and asked if i fell asleep. I kinda looked embarrased and laughed in a silly way at him. My gosh why does this happen to me. The same thing happened before after lunch, but it was Dr Lamb instead. He also gave me a big smile and asked about my project. Told him it was going wrong and he replied that his second year results were good. I remarked how long that was, (actually it was only last year) and that i have gotten really old. He replied, "yes! You are 21!! I remember!" WHOA!!! That was really scary. So i laughed and said to him, yes i am getting really old. He said "yes you were all so young and innocent before." Ok, that was starting to get freaky....i kinda laughed and he left, but not before coming back and saying, "At least i'm much ahead in years, so you are fine in comparison!" .............speechless*..............
Sooooo today was another horrible day, i'm tired, restless, depressed and i banged my head on the table too! sniff* sniff*
Highlight of the day, i got a phone call from my lovely friend Naomi. After being comforted, i felt my day wasn't so bad afterall!!!
Got to uni and the first thing i knew was that i had to move upstairs to do my experiments but wingkit got to stay cos he got there before me! GRRRRR!!!! poo u wingkit. So, after wasting 30 min getting my stuff upstairs and to get used to the surroundings i found that it was not so bad after all. I get to have my own spectophotometer, there were three centrifuge machines, lots of pipettes and lots of SPACES!!!! hehe, the only thing it lacked was the company. Ah well, to make it up, i went downstairs to get some more stuff but also to make Wingkit really guilty. Hehe, and i succeeded, mwuhahahaha!!!
Awww, but then i felt bad so i stopped and went back upstairs again to face the stupid method. I did the assay i did yesterday and there was no reaction at all!!! It was 12 then and was ordered by Derek to go have lunch and do another method in the afternoon. So i trouped off with Wingkit to have lunch outside. Wow, it was really warm. I went to have ice-cream as well. Tried to coax Wingkit to have some too, cos i was feeling like a pig, but that did not work. I also noticed how thin he actually is when he keeps complaining how FAT he is. Ok, if he is "fat", then i must be OBESE!!!!
After chatting to Chin and Basma for a few minutes, we went back upstairs to face our project again. groan*
This time i did the GST binding buffer assay. Purifying the protein from the bacteria and then react it with the substrate (dichrlor stuff) and glutathione. I did the reaction and then found out that it did not work either. I also managed to bugger up the concentration/volume/measurement/counting/timing of the stupid method. Sigh, dunno if it really mess my results that bad or not. Then after Derek came up to check up on me, i realised something....i had forgotten to put the glutathione into the Reconstitution buffer!!!! CRAP CRAP CRAP CRAP CRAP CRAP CRAP*
I spent the last hour redoing most of the method which includes long minutes of contrifuging and shaking the mixture for ages. I got so sleepy that i put my head on the table and shook the tube in one hand. Just at that point i heard the door open and Dr Archer came through the door. I bolted upright and gave a sheepish smile. He laughed at me and asked if i fell asleep. I kinda looked embarrased and laughed in a silly way at him. My gosh why does this happen to me. The same thing happened before after lunch, but it was Dr Lamb instead. He also gave me a big smile and asked about my project. Told him it was going wrong and he replied that his second year results were good. I remarked how long that was, (actually it was only last year) and that i have gotten really old. He replied, "yes! You are 21!! I remember!" WHOA!!! That was really scary. So i laughed and said to him, yes i am getting really old. He said "yes you were all so young and innocent before." Ok, that was starting to get freaky....i kinda laughed and he left, but not before coming back and saying, "At least i'm much ahead in years, so you are fine in comparison!" .............speechless*..............
Sooooo today was another horrible day, i'm tired, restless, depressed and i banged my head on the table too! sniff* sniff*
Highlight of the day, i got a phone call from my lovely friend Naomi. After being comforted, i felt my day wasn't so bad afterall!!!

4 Comments:
Poo you for making me feel guilty!!
Well i have ice cream EVERYDAY!!! So i would be the pig if i had two helpings!!
Still think i am fat.....
Lol, SA is a cool guy! Just dont really want him marking my work (and thankfully he isnt!) Lamb is just scaring me more and more..... although as a lecturer he is nice.... just some of his comments!
By
Wink, at 3:17 PM
Of all people Lamb came in!! Lol.
By
XiYu the Great, at 3:22 PM
Lol, i agree, he is a nice lecturer but he does have weird comments.
lol, well both Lamb and Archer came in and both caught me sleeping!!
By
Anoa hime, at 3:28 PM
Ha ha ha!!! How funny. Can't say that I'm a Lamb fan. But I don't mind Archer. Tough marker, but swell guy. Right on!
By
XiYu the Great, at 1:37 AM
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